The viable but non-culturable (VBNC) state in pathogenic Escherichia coli: A general review

: 2 credits
Expiry Date
: None
CPD Units
: 2.00 Points
: Not yet rated.

This review discusses various general aspects of the VBNC state, and the mechanisms and possible public health impact of indicator and pathogenic E. coli entering into the VBNC state.

Keywords: Viable but non-culturable (VBNC), Escherichia coli (E. coli), cell viability, gene expression, resuscitation, virulence

Reading material:

Educational Objectives

Educational Aim:
The aim of this module is to provide an overview of the viable but non-culturable state, with particular reference to pathogenic E. coli and the possible public health implications.

Editor's comments:
Pathogenic and indicator E. coli entering into the VBNC state could have an adverse effect on public health in S.A. (and other parts of the world) as the conventional food and water safety checks involve culturability, which may mask the potential number of non-culturable viable cells that have the potential to cause disease upon resuscitation..

Educational Outcomes:
Upon completion of this module practitioners should have a clear understanding of:

  1. The viable but non-culturable state (VBNC) state.
  2. . coli in the VBNC state.
  3. The public health implications of pathogenic E. coli entering into the VBNC state.

Instruction for this Module

  • Read the supplied reading material and complete the quiz at the end
  • You need to pass the quiz and complete the course rating to generate the module certificate at the end
  • You have three attempts to pass the quiz
  • The pass grade is 70%
  • Please click on the CPD certificate link to claim your certificate and to update your CPD Manager

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